Hb Tochigi	beta56(D7)-beta59(E3) Gly-Asn-Pro-Lys->0

HEMATOLOGY		Chronic hemolytic anemia
ELECTROPHORESIS		Hb X moves ahead of Hb A at alkaline pH; Hb X moves faster to the anode than Hb A at acidic pH
CHROMATOGRAPHY		Not reported
STRUCTURE STUDIES		pCMB; tryptic digestion; separation of peptides by fingerprinting; amino acid analysis
DNA ANALYSES		Not done
FUNCTION STUDIES		Not reported; metHb
STABILITY		Unstable
OCCURRENCE		Found in three members of a Japanese family
OTHER INFORMATION		Quantity in the heterozygote 20-25%

REFERENCES
1.		Shibata, S., Miyaji, T., Ueda, S., Matsuoka, M., Iuchi, I., Yamada, K., and Shinkai, N.: Proc. Jpn. Acad., 46:440, 1970.
2.		Yamada, K., Shinkai, N., Nakazawa, S., Yamada, Z., Saito, K., Miwa, S., Miyaji, T., and Shibata, S.: Acta Haematol. Jap., 34:484, 1971.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.