Hb Freiburg	beta23(B5)Val->0

HEMATOLOGY		Mild hemolytic anemia with cyanosis in the heterozygote
ELECTROPHORESIS		Hb X moves in the position of Hb F in starch gel electrophoresis at alkaline pH
CHROMATOGRAPHY		Hb X and Hb A separate in both cation and anion exchange HPLC
STRUCTURE STUDIES		Tryptic digestion of AE-betaX chain; separation of peptides by cation exchange chromatography; amino acid analysis
DNA ANALYSES		Not done
FUNCTION STUDIES		Increased oxygen affinity; metHb with specific spectrum
STABILITY		Unstable
OCCURRENCE		Found in members of a German family
OTHER INFORMATION		Quantity in heterozygotes 27-32%

REFERENCES
1.		Betke, K. and Kleihauer, E.: Schweiz. Med. Wschr., 42:1316, 1962.
2.		Jones, R.T., Brimhall, B., Huisman, T.H.J., Kleihauer, E., and Betke, K.: Science, 154:1024, 1966.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.