Hb F-Forest Park	Agamma73(E17)Asp->Asn; 75(E19)Ile->Thr

ELECTROPHORESIS		Hb FX moves between Hb S and Hb C at alkaline pH
IEF		Not reported
CHROMATOGRAPHY		Hb FX eluted between Hb A2 and Hb A in DEAE-cellulose chromatography
CHAIN SEPARATION		AgammaX = 18.3%; Ggamma = 70.3%; Agamma = 11.4%
STRUCTURE STUDIES		Tryptic digestion of gammaX chain; separation of peptides by reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES		A GAT->AAT mutation at codon 73; confirmed by gene mapping
NOTES		Found in Caucasian newborn babies; Hb FX 12.2% of total Hb

REFERENCES
1.		Chen, S-S., Webber, B.B., Wilson, J.B., and Huisman, T.H.J.: Biochim. Biophys. Acta, 832:242, 1985.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.