Hb Montreal	Deletion of Asp-Gly-Leu- at beta73, beta74, beta75;
	insertion of Ala-Arg-Cys-Gln-

HEMATOLOGY		Moderately severe hemolytic anemia; Heinz bodies; reticulocytosis
ELECTROPHORESIS		Hb X moves like Hb D in IEF
CHROMATOGRAPHY		Hb X elutes much slower than Hb A2 on a cation exchange HPLC column; the betaX chain elutes ahead of betaA (partially separated) on a reversed phase HPLC column
STRUCTURE STUDIES		Tryptic digestion of AE-betaX chain; separation of peptides by reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES		Modified sequence determined between codons 75 and 77 of the beta gene; see original paper for details
FUNCTION STUDIES		Not done
STABILITY		Unstable
OCCURRENCE		Found in a 7-year-old French-Canadian male but not in his parents
OTHER INFORMATION		Hb X 15-20% of total Hb

REFERENCES
1.		Plaseska, D., Dimovski, A.J., Wilson, J.B., Webber, B.B., Hume, H.A., and Huisman, T.H.J.: Blood, 77:178, 1991.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.