Hb Grady	Glu-Phe-Thr- inserted between
	alpha118(H1)Thr and alpha119(H2)Pro

ALSO KNOWN AS		Dakar
HEMATOLOGY		Normal in the heterozygote
ELECTROPHORESIS		Hb X moves faster than Hb A at alkaline pH
CHROMATOGRAPHY		Hb X was isolated by DEAE-Sephadex and DEAE-cellulose chromatography
STRUCTURE STUDIES		Tryptic digestion; separation of peptides by cation exchange chromatography or fingerprinting; amino acid analysis; sequencing
DNA ANALYSES		Not reported; mismatched intragenic crossover suggested
FUNCTION STUDIES		Nearly normal oxygen affinity; Bohr effect and cooperativity normal
STABILITY		Unstable
OCCURRENCE		Found in a few Black families
OTHER INFORMATION		Quantity in the heterozygote 8%; found in association with alpha-thal

REFERENCES
1.		Huisman, T.H.J., Wilson, J.B., Gravely, M., and Hubbard, M.: Proc. Natl. Acad. Sci. USA, 71:3270, 1974.
2.		Huisman, T.H.J. and Miller, A.: Am. J. Hum. Genet., 28:363, 1976.
3.		Garel, M.C., Goossens, M., Oudart, J.L., Blouquit, Y., Thillet, J., and Rosa, J.: Biochim. Biophys. Acta, 453:459, 1976.
4.		Scott, A.F., Phillips, J.A., III, Young, K.E., Kazazian, H.H., Jr., Smith, K.D., Charache, S., and Clegg, J.B.: Am. J. Hum. Genet., 33:129, 1981.
5.		Cleek, M.P., Gardiner, M.B., Reese, A.L., Harris, H.F., Felice, A.E., and Huisman, T.H.J.: Am. J. Hum. Genet., 35:1314, 1983.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.