Hb Lepore-Boston-Washington	deltabeta hybrid (delta through 87; beta from 116)

HEMATOLOGY		Mild anemia with microcytosis and hypochromia in the heterozygote (beta+-thal)
ELECTROPHORESIS		Hb X and Hb A can be separated by standard procedures at alkaline pH; Hb X moves to the position of Hb S
CHROMATOGRAPHY		Hb X and Hb A can be separated by cation and anion exchange chromatography; it elutes between Hb A and Hb A2 in cation exchange HPLC
STRUCTURE STUDIES		Tryptic digestion; separation of peptides by fingerprinting and cation exchange chromatography; amino acid analysis; sequencing
DNA ANALYSES		A large deletion was observed by gene mapping
FUNCTION STUDIES		Not reported
STABILITY		Stable
OCCURRENCE		Hb Lepore-B-W is the most common Hb Lepore type; found mainly in Italian families; it has also been observed in families from Rumania, Yugoslavia, Turkey, Cyprus, Jamaica, Cuba, Greece, England, Australia, Mexico, etc.
OTHER INFORMATION		Quantity in heterozygotes 7-13%; found in combination with Hb S, Hb C, beta+-thal, and in the homozygous state

REFERENCES
1.		Baglioni, C.: Proc. Natl. Acad. Sci. USA, 48:1880, 1962.
2.		Labie, D., Schroeder, W.A., and Huisman, T.H.J.: Biochim. Biophys. Acta, 127: 428, 1966.
3.		And numerous others. The reader is invited to consult text books such as: Bunn and Forget, Hemoglobin: Molecular, Genetic and Clinical Aspects, Saunders, Philadelphia, 1986; Weatherall and Higgs, The Haemoglobinopathies, Bailliere's Clinical Haematology, Vol. 6, Saunders, London, 1993.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.