Hb F-Sacromonte	Ggamma59(E3)Lys->Gln

ALSO KNOWN AS		F-Foch
ELECTROPHORESIS		Hb FX moves faster than Hb A at alkaline pH
IEF		Same results
CHROMATOGRAPHY		No separation by cation exchange HPLC
CHAIN SEPARATION		GgammaX = 31% of total gamma; AgammaT = 16.4%; Ggamma = 31.5%; Agamma = 21.1%
STRUCTURE STUDIES		Tryptic digestion of AE-gammaX chain; separation of peptides by reversed phase HPLC; amino acid analysis
DNA ANALYSES		An AAA->CAA mutation at codon 59
NOTES		Found in a Spanish newborn, and in a French baby; Hb FX 25% of total Hb

REFERENCES
1.		Pobedimskaya, D.D., Molchanova, T.P., Gu, L-H., Molina, M.A., de Pablos, J.Ma., and Huisman, T.H.J.: Hemoglobin, 17:269, 1993.
2.		Abbes, S., Fitzgerald, P.A., Varady, E., Girot, R., Pic, P., Blouquit, Y., Ducrocq, R., Drupt, F., and Wajcman, H.: Hemoglobin, 19:173, 1995.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.