Hb F-Port Royal	Ggamma125(H3)Glu->Ala

ELECTROPHORESIS		Hb FX moves between Hb S and Hb C
IEF		The same
CHROMATOGRAPHY		Hb FX can be separated by cation and anion exchange HPLC
CHAIN SEPARATION
STRUCTURE STUDIES		Tryptic digestion of gammaX chain; separation of peptides by cation exchange HPLC; amino acid analysis; glycinamida-tion; sequencing
DNA ANALYSES		Not reported; presumed mutation GAG->GCG at codon 125
NOTES		Found in Black babies; associated with the Ggamma-Ggamma arrangement; quantity of Hb FX 14-16% of total Hb F

REFERENCES
1.		Brimhall, B., Vedvick, T.S., Jones, R.T., Ahern, E., Palomino, E., and Ahern, V.: Br. J. Haematol., 27:313, 1974.
2.		Huisman, T.H.J.: Personal communication, 1983.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.