Hb F-M-Fort Ripley	Ggamma92(F8)His->Tyr

ELECTROPHORESIS		No separation observed
IEF		No separation observed
CHROMATOGRAPHY		No separation observed
CHAIN SEPARATION		GgammaX = 12.4% of total gamma; AgammaT = 22.7%; Ggamma = 45.1%; Agamma = 19.8%
STRUCTURE STUDIES		Tryptic digestion of Hb FX; separation of peptides by reversed phase HPLC; amino acid analysis
DNA ANALYSES		A CAC->TAC mutation at codon 92 (Ref. 3)
NOTES		Found in an American and a Canadian newborn; neonatal cyanosis; spectral changes are described in Ref. 3; slightly increased oxygen affinity

REFERENCES
1.		Priest, J.R., Watterson, J., Jones, R.T. Faassen, A.E., and Hedlund, B.E.: Pediatrics, 83:734, 1989.
2.		Glader, B.E.: Pediatrics, 83:792, 1989.
3.		Molchanova, T.P., Wilson, J.B., Gu, L-H., Hain, R.D.W., Chang, L.S., Poon, A.O., and Huisman, T.H.J.: Hemoglobin, 16:389, 1992.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.