Hb F-Catalonia	Ggamma15(A12)Trp->Arg

ELECTROPHORESIS		Hb FX moves slower than Hb F (between Hb S and Hb C) at alkaline pH
IEF		Hb FX focuses between Hb S and Hb C
CHROMATOGRAPHY		Hb FX can readily be separated from Hb A and Hb F by DEAE-cellulose chromatography
CHAIN SEPARATION		GgammaX = 36.8%; Ggamma = 35.4%; Agamma = 27.8%; also see NOTES
STRUCTURE STUDIES		Tryptic digestion of gammaX chain; separation of peptides by reversed phase HPLC; amino acid analysis
DNA ANALYSES		Not reported; presumed mutation TGG->CGG at codon 15
NOTES		Found in two newborn babies from Northern Spain; the sec-ond baby was also an AgammaT heterozygote (Agamma75 Ile->Thr): AgammaT 11.5%; GgammaX 45.5%; Ggamma 31.3%; Agamma 11.7%

REFERENCES
1.		Plaseska, D., Wilson, J.B., Kutlar, F., Font, Ll., Baiget, M., and Huisman, T.H.J.: Hemoglobin, 14:511, 1990.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.