Hb F-Carlton	Ggamma121(GH4)Glu->Lys

ELECTROPHORESIS		Hb FX moves slower than Hb C at alkaline pH
IEF		About the same
CHROMATOGRAPHY		Hb FX can be separated from Hb A and Hb F by DEAE-cellulose chromatography
CHAIN SEPARATION
STRUCTURE STUDIES		Tryptic digestion of isolated gammaX chain; separation of pep-tides by fingerprinting; amino acid analysis
DNA ANALYSES		Not reported; presumed mutation GAA->AAA at codon 121
NOTES		Found in a newborn of Italian parentage; Hb FX 26% of total Hb F

REFERENCES
1.		Brennan, S.O., Smith, M.B., and Carrell, R.W.: Biochim. Biophys. Acta, 490:452, 1977.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.