Hb F-Brooklyn	Ggamma66(E10)Lys->Gln

ELECTROPHORESIS		Hb FX moves slightly faster than Hb A at alkaline pH
IEF		About the same
CHROMATOGRAPHY		Hb FX can be separated from Hb A and Hb F by cation exchange HPLC
CHAIN SEPARATION		GgammaX = 25.1% of total gamma; Ggamma = 39.3%; Agamma = 35.6%
STRUCTURE STUDIES		Tryptic digestion of gammaX chain; separation of peptides by reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation AAG->CAG at codon 66
NOTES		Found in a Caucasian newborn; Hb FX 23% of total Hb

REFERENCES
1.		Plaseska, D., Li, H-J., Wilson, J.B., Kutlar, F., Kutlar, A., Huisman, T.H.J., and Kulpa, J.: Hemoglobin, 14:213, 1990.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.