Hb Tak	beta147 (+AC); abnormal C-terminal sequence:
	(147)THR-Lys-Leu-Ala-Phe-Leu-Leu-
	Ser-Asn-Phe-(157)Tyr-COOH

HEMATOLOGY		Nearly normal in the heterozygote
ELECTROPHORESIS		Hb X moves to the position of Hb G at alkaline pH; at acidic pH Hb X moves as Hb S
CHROMATOGRAPHY		Hb X was isolated on a DEAE-Sephadex column
STRUCTURE STUDIES		Tryptic digestion; separation of peptides by fingerprinting; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed frameshift at terminating codon beta147 (+AC)
FUNCTION STUDIES		Increased oxygen affinity; decreased Bohr effect and cooperativity
STABILITY		Heat stable but mildly unstable with the isopropanol test
OCCURRENCE		Found in a few Thai families
OTHER INFORMATION		Quantity in the heterozygote 35-40%; found in association with beta-thal

REFERENCES
1.		Flatz, G., Kinderlerer, J.L., Kilmartin, J.V., and Lehmann, H.: The Lancet, i:732, 1971.
2.		Imai, K. and Lehmann, H.: Biochim. Biophys. Acta, 412:288, 1975.
3.		Lehmann, H., Casey, R., Lang, A., Stathopoulou, R., Imai, K., Tuchinda, S., Vinai, P., and Flatz, G.: Br. J. Haematol., 31:119 (Suppl.), 1975.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.