Hb South Florida	beta1(NA1)Val->Met; amino terminus extended with a
	methionyl residue: (-1)Met-(+1)Met-(+2)His-Leu-

HEMATOLOGY		Probably normal
ELECTROPHORESIS		Hb X focuses ahead of Hb A in IEF
CHROMATOGRAPHY		Hb X elutes with Hb A1c in cation exchange HPLC
STRUCTURE STUDIES		Tryptic digestion of AE-betaX chain; separation of peptides by reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation GTG->ATG at codon beta1; this prevents the removal of the initiator methionine
FUNCTION STUDIES		Not reported
STABILITY		Not reported
OCCURRENCE		Found in members of a Caucasian family
OTHER INFORMATION		Quantity in the heterozygote 12-20%; about 20% of the variant is acetylated

REFERENCES
1.		Boissel, J-P., Kasper, T.J., Shah, S.C., Malone, J.I., and Bunn, H.F.: Proc. Natl. Acad. Sci. USA, 82:8448, 1985.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.