Hb A2-Sphakia	delta2(NA2)His->Arg

CONTACT		2,3-DPG binding site
ELECTROPHORESIS		Hb X moves slower than Hb A2 but faster than Hb B2 (see delta16) at alkaline pH
IEF		Not reported
CHROMATOGRAPHY		Hb X was isolated by DEAE-Sephadex chromatography; Hb X elutes ahead of Hb A2
STRUCTURE ANALYSIS		Tryptic digestion of AE-deltaX chain; peptides separated by chromatography; amino acid analysis
DNA ANALYSIS		Not reported; presumed mutation CAC->CGC at codon 2
NOTES		Found in families on Crete and in Canada (Indians)

REFERENCES
1.		Jones, R.T., Brimhall, B., Huehns, E.R., and Barnicot, N.A.: Science, 151:1406, 1966.
2.		Vella, F., Wong, S.C., Wilson, J.B., and Huisman, T.H.J.; Can. J. Biochem., 50:841, 1972.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.