Hb A2-Flatbush	delta22(B4)Ala->Glu

CONTACT		External
ELECTROPHORESIS		Hb X moves in the position of Hb S at alkaline pH
IEF		Same
CHROMATOGRAPHY		Hb X can be isolated by DEAE cellulose chromatography; Hb X elutes after Hb A2
STRUCTURE ANALYSIS		Tryptic digestion of deltaX chain; separation of peptides by cation exchange HPLC; amino acid analysis; sequencing
DNA ANALYSIS		Not reported; presumed mutation GCA->GAA at codon 22
NOTES		Found in a few Black families

REFERENCES
1.		Ranney, H.M., Jacobs, A.S., Bradley, T.B., and Cordova, F.A.: Nature, 197:164, 1963.
2.		Lee, R.C. and Huisman, T.H.J.: Blood, 24:495, 1964.
3.		Jones, R.T., Brimhall, B., and Huisman, T.H.J.: J. Biol. Chem., 242:5141, 1967.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.