Hb A2-Canada	delta99(G1)Asp->Asn

CONTACT		Internal; near cavity; alpha2beta1 contact
ELECTROPHORESIS		Hb X moves slower than Hb A2 at alkaline pH; Hb X cannot be separated from Hb B2 (delta16)
IEF		Not reported
CHROMATOGRAPHY		Hb X was isolated by DEAE-cellulose chromatography; Hb X elutes ahead of Hb A2
STRUCTURE ANALYSIS		Tryptic digestion of AE-deltaX chain; separation of peptides by cation exchange HPLC; amino acid analysis; sequencing
DNA ANALYSIS		Not reported; presumed mutation GAT->AAT at codon 99
NOTES		Found in an Indian family; occurred in association with beta-thal; Hb X has an increased affinity for oxygen

REFERENCES
1.		Salkie, M.L., Gordon, P.A., Rigal, W.M., Lam, H., Wilson, J.B., Headlee, M.E., and Huisman, T.H.J.: Hemoglobin, 6:223, 1982.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.