Hb A2' (or B2)	delta16(A13)Gly->Arg

CONTACT		External
ELECTROPHORESIS		Hb X moves slower than Hb A2 at alkaline pH
IEF		Same
CHROMATOGRAPHY		Hb X can be separated from Hb A and Hb A2 by cation and anion exchange chromatography
STRUCTURE ANALYSIS		Tryptic digestion of deltaX chain; separation of peptides by fingerprinting, cation exchange chromatography or reversed phase HPLC; amino acid analysis
DNA ANALYSIS		A GGC->CGC mutation at codon 16
NOTES		Hb A2' is the most common delta chain variant; it is found mainly in Black families; it is observed in heterozygotes, homozygotes, in combination with Hb S, Hb C, beta-thal (in cis and in trans)

REFERENCES
1.		Horton, B.F., Payne, R.A., Bridges, M.T., and Huisman, T.H.J.: Clin. Chim. Acta, 6:246, 1961.
2.		Huisman, T.H.J., Punt, K., and Schaad, J.D.G.: Blood, 17:747, 1961.
3.		Pearson, H.A. and Moore, M.M.: Am. J. Hum. Genet., 17:125, 1965.
4.		Ball, E.W., Meynell, M.J., Beale, D., Kynoch, P., Lehmann, H., and Stretton, A.O.W.: Nature, 209:1217, 1966.
5.		Jones, R.T., Brimhall, B., and Huisman, T.H.J.: J. Biol. Chem., 242:5141, 1967.
6.		Codrington, J.F., Li, H-W., Kutlar, F., Gu, L-H., Ramachandran, M., and Huisman, T.H.J.: Blood, 76:1246, 1990.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.