Hb Wood	beta97(FG4)His->Leu

CONTACT		External; alpha1beta2 contact
HEMATOLOGY		Erythrocytosis in the heterozygote
ELECTROPHORESIS		No separation of Hb X and Hb A by standard techniques; separation is possible by IEF
CHROMATOGRAPHY		Hb X can be isolated by cation exchange chromatography
STRUCTURE STUDIES		Tryptic digestion of AE-betaX chain; separation of peptides by fingerprinting; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation CAC->CTC at codon 97
FUNCTION STUDIES		Increased oxygen affinity; decreased cooperativity; normal Bohr effect
STABILITY		Normal
OCCURRENCE		Found in a family of Swedish-Norwegian descent
OTHER INFORMATION		Quantity in the heterozygote ~50%

REFERENCES
1.		Taketa, F., Huang, Y.P., Libnoch, J.A., and Dessel, B.H.: Biochim. Biophys. Acta, 400:348, 1975.
2.		Taketa, F., Antholine, W.E., Mauk, A.G., and Libnoch, J.A.: Biochemistry, 14: 3229, 1975.

---

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.