Hb Rio Grande	beta8(A5)Lys->Thr

CONTACT		External
HEMATOLOGY		Normal in the heterozygote
ELECTROPHORESIS		Hb X moves between Hb A and Hb J on cellulose acetate at alkaline pH and between Hb A and Hb F on citrate agar at acidic pH
CHROMATOGRAPHY		Not reported
STRUCTURE STUDIES		Tryptic digestion; fingerprinting; reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation AAG->ACG at codon 8
FUNCTION STUDIES		Normal oxygen affinity, 2,3-DPG, and IHP
STABILITY		Normal
OCCURRENCE		Found in members of a Mexican-American family
OTHER INFORMATION		Quantity in the heterozygote 48.1%

REFERENCES
1.		Moo-Penn, W.F., Johnson, M.H., McGuffey, J.E., Jue, D.L., and Therrell, B.L., Jr.: Hemoglobin, 7:91, 1983.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.