Hb Nagoya	beta97(FG4)His->Pro

CONTACT		External; alpha1beta2 contact
HEMATOLOGY		Chronic hemolytic anemia in the heterozygote
ELECTROPHORESIS		Hb X focuses slightly more anodic than Hb A by IEF
CHROMATOGRAPHY		The betaX chain coeluted with the alphaA chain in reversed phase HPLC
STRUCTURE STUDIES		pCMB precipitation; tryptic digestion of AE-betaX chain; separation of peptides by reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation CAC->CCC at codon 97
FUNCTION STUDIES		Increased oxygen affinity; decreased cooperativity
STABILITY		Unstable
OCCURRENCE		Found in members of a Japanese family
OTHER INFORMATION		Quantity in the heterozygote 16.7 and 25.5%; variant susceptible to oxidation; it loses heme during that process

REFERENCES
1.		Ohba, Y., Imanaka, M., Matsuoka, M., Hattori, Y., Miyaji, T., Funaki, C., Shibata, S., Shimokata, H., Kuzuya, F., and Miwa, S.: Hemoglobin, 9:11, 1985.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.