Hb N-Seattle	beta61(E5)Lys->Glu

CONTACT		External
HEMATOLOGY		Normal in the heterozygote
ELECTROPHORESIS		Hb X and Hb A separate readily at alkaline pH (Hb X moves faster than Hb A) but not at acidic pH
CHROMATOGRAPHY		Not reported
STRUCTURE STUDIES		Tryptic digestion; separation of peptides by fingerprinting and cation exchange chromatography; amino acid analysis; leucine amino peptidase; Edman degradation
DNA ANALYSES		Not reported; presumed mutation AAG->GAG at codon 61
FUNCTION STUDIES		Not reported
STABILITY		Normal
OCCURRENCE		Found in a Black male who was a blood donor
OTHER INFORMATION		Quantity in the heterozygote ~50%

REFERENCES
1.		Jones, R.T., Brimhall, B., Huehns, E.R., and Motulsky, A.G.: Biochim. Biophys. Acta, 154:278, 1968.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.