Hb J-Cairo	beta65(E9)Lys->Gln

CONTACT		External
HEMATOLOGY		Slight hypochromic microcytic anemia in the heterozygote
ELECTROPHORESIS		Hb X and Hb A separate at alkaline pH (Hb X is faster than Hb A) but not at acidic pH; excellent separation by IEF
CHROMATOGRAPHY		Hb X was isolated by DEAE-Sephadex chromatography
STRUCTURE STUDIES		Tryptic digestion; separation of peptides by fingerprinting or cation exchange chromatography; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation AAG->CAG at codon 65
FUNCTION STUDIES		Decreased oxygen affinity and cooperativity; normal Bohr effect
STABILITY		Normal; increased auto-oxidation
OCCURRENCE		Found in an Egyptian female and her father
OTHER INFORMATION		Quantity in heterozygotes 40-45%

REFERENCES
1.		Garel, M.C., Hassan, W., Coquelet, M.T., Goossens, M., and Rosa, J.: Biochim. Biophys. Acta, 420:97, 1976.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.