Hb I-Toulouse	beta66(E10)Lys->Glu

CONTACT		Heme contact
HEMATOLOGY		Mild chronic hemolytic anemia in the heterozygote
ELECTROPHORESIS		Hb X and Hb A can be separated at alkaline pH; Hb X moves considerably faster than Hb A
CHROMATOGRAPHY		Hb X was isolated by DEAE-Sephadex chromatography
STRUCTURE STUDIES		Tryptic digestion; separation of peptides by fingerprinting and cation exchange chromatography; amino acid analysis; Edman degradation
DNA ANALYSES		Not reported; presumed mutation AAA->GAA at codon 66
FUNCTION STUDIES		Normal
STABILITY		Unstable; ferriHb
OCCURRENCE		Found in a French male, a Nicaraguan female in association with Hb S, and in Solomon Islanders in association with alpha-thal
OTHER INFORMATION		Quantity in heterozygotes 33-40% (lowest value in the subjects with alpha-thal); tendency to form metHb

REFERENCES
1.		Rosa, J., Labie, D., Wajcman, H., Boigne, J.M., Cabannes, R., Bierme, R., and Ruffie, J.: Nature, 223:190, 1969.
2.		Labie, D., Rosa, J., Belkhodja, O., and Bierme, R.: Biochim. Biophys. Acta, 236:201, 1971.
3.		Hendy, J.G. and Cauchi, M.N.: Hemoglobin, 18:227, 1994.

---

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.