Hb Hotel-Dieu	beta99(G1)Asp->Gly

CONTACT		Internal; alpha1beta2 contact
HEMATOLOGY		Erythrocytosis in the heterozygote (PCV 0.42-0.63 l/l)
ELECTROPHORESIS		Hb X migrates slightly slower than Hb F at alkaline pH
CHROMATOGRAPHY		Hb X was isolated by DEAE-Sephacel chromatography; Hb X elutes free of Hb A2 and Hb A
STRUCTURE STUDIES		Tryptic digestion; separation of peptides by fingerprinting; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation GAT->GGT at codon 99
FUNCTION STUDIES		Increased oxygen affinity; decreased cooperativity
STABILITY		Not reported
OCCURRENCE		Found in three members of a Caucasian French family
OTHER INFORMATION		Quantity in the heterozygote 38-48%

REFERENCES
1.		Blouquit, Y., Braconnier, F., Galacteros, F., Arous, N., Soria, J., Zittoun, R., and Rosa, J.: Hemoglobin, 5:19, 1981.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.