Hb D-Granada	beta22(B4)Glu->Val

CONTACT		External
HEMATOLOGY		Normal in the heterozygote
ELECTROPHORESIS		Hb X and Hb A separate at alkaline pH; Hb X moves about as Hb S
CHROMATOGRAPHY		Hb X and Hb A separate in cation and in anion exchange chromatography
STRUCTURE STUDIES		Tryptic digestion; separation of peptides on a reversed phase HPLC column; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation GAA->GTA at codon 22
FUNCTION STUDIES		Not reported
STABILITY		Not reported
OCCURRENCE		Found in a Gypsy family in Granada, Spain
OTHER INFORMATION		Quantity in the heterozygote 42.6%

REFERENCES
1.		de Pablos, J.Ma., Kutlar, A., Wilson, J.B., Webber, B.B., Hu, H., and Huisman, T.H.J.: Hemoglobin, 11:563, 1987.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.