Hb Chico | beta66(E10)Lys->Thr |
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CONTACT | Heme contact | ||
HEMATOLOGY | Mild anemia in the heterozygote | ||
ELECTROPHORESIS | Hb X and Hb A separate at alkaline pH (Hb X has the mobility of Hb J) but not at acidic pH | ||
CHROMATOGRAPHY | Incomplete separation of Hb X and Hb A by cation exchange HPLC; good separation by DEAE-Sephadex chromatography; betaA, betaX, and alpha chains separate in reversed phase HPLC; elution in the order listed | ||
STRUCTURE STUDIES | Tryptic digestion of betaX chain; separation of peptides by reversed phase HPLC; amino acid analysis | ||
DNA ANALYSES | Not reported; presumed mutation AAA->ACA at codon 66 | ||
FUNCTION STUDIES | Decreased oxygen affinity; normal cooperativity | ||
STABILITY | Slightly unstable | ||
OCCURRENCE | Found in a family living in Chico, California, USA | ||
OTHER INFORMATION | Quantity in the heterozygote ~45% |
REFERENCES | |||
1. | Shih, D.T-B., Jones, R.T., Shih, M.F-C., Jones, M.B., Koler, R.D., and Howard, J.: Hemoglobin, 11:453, 1987. |