Hb Beckman	beta135(H13)Ala->Glu
	(or perhaps Asp)

NOTE:		An Ala->Glu change would require a GCA or GCG mutation to GAA or GAG. The codons GCA and GCG do not occur in the beta-globin gene; GCA is in the gamma gene and GCG in the alpha gene
CONTACT		Central cavity
HEMATOLOGY		Chronic anemia; microcytosis; reticulocytosis
ELECTROPHORESIS		Hb X and Hb A can be separated at alkaline pH; Hb X moves faster than Hb A
CHROMATOGRAPHY		Not reported; the betaX and betaA chains separate by reversed phase HPLC (elution order: betaA, betaX, alpha)
STRUCTURE STUDIES		Tryptic digestion of betaX chain; analysis of tryptic peptides by reversed phase HPLC; amino acid analysis; FAB mass spectrometry
DNA ANALYSES		Not reported; presumed mutation GCT->GAT at codon 135 that corresponds to an Ala->Asp replacement
FUNCTION STUDIES		Decreased oxygen affinity
STABILITY		Unstable
OCCURRENCE		Found in a Black female
OTHER INFORMATION		None

REFERENCES
1.		Rahbar, S., Lee, T., and Asmeron, Y.: Blood, 78:204a (Suppl. 1), 1991.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.