Hb Agenogi	beta90(F6)Glu->Lys

CONTACT		External
HEMATOLOGY		Normal in the heterozygote
ELECTROPHORESIS		Hb X and Hb A separate at alkaline pH; Hb X moves close to Hb A2
CHROMATOGRAPHY		Hb X elutes behind Hb A2 from a cation exchange HPLC column
STRUCTURE STUDIES		Tryptic digestion; separation of peptides by fingerprinting; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation GAG->AAG at codon 90
FUNCTION STUDIES		Slightly reduced oxygen affinity; normal Bohr effect and cooperativity
STABILITY		Slightly reduced
OCCURRENCE		Found in a Japanese family, a Black American family, and a Hungarian family
OTHER INFORMATION		Quantity in the heterozygote 36.5-40.5%; occurred in association with a betao-thal

REFERENCES
1.		Miyaji, T., Suzuki, H., Ohba, Y., and Shibata, S.: Clin. Chim. Acta, 14:624, 1966.
2.		Imai, K., Morimoto, H., Kotani, M., Shibata, S., Miyaji, T., and Matsutomo, K.: Biochim. Biophys. Acta, 200:197, 1970.
3.		Castro, O., Winter, W.P., Doan, R.J., Lee, C.K., and Rucknagel, D.L.: Clin. Res., 24:630A, 1976.
4.		Corso, D., Cognata, B., Ciaccio, C., Piazza, T., Dibenedetto, S.P., Samperi, P., Russo Mancuso, G., and Schiliro, G.: Hemoglobin, 14:549, 1990.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.