Hb J-Singa	alpha78(EF7)Asn->Asp

CONTACT		External
HEMATOLOGY		Normal in the heterozygote
ELECTROPHORESIS		The fast-moving Hb X separates from Hb A at alkaline pH
CHROMATOGRAPHY		Hb X and Hb A were separated by DEAE-cellulose chromatography
STRUCTURE STUDIES		Tryptic peptides were separated by reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation AAC->GAC; alpha2 or alpha1
FUNCTIONAL STUDIES		Not reported
STABILITY		Normal
OCCURRENCE		Found in a French-Acadian family living in Canada
OTHER INFORMATION		Quantity in the heterozygote 25-32% (alpha-thal-2 was not excluded)

REFERENCES
1.		Wong, S.C., Ali, M.A.M., Pond, J.R., Rubin, S.M., Johnson, S.E.N., Wilson, J.B., and Huisman, T.H.J.: Biochim. Biophys. Acta, 784:187, 1984.

---

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.