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ALSO KNOWN AS | |
Aida | |
CONTACT | |
External | |
HEMATOLOGY | |
Normal in the heterozygote | |
ELECTROPHORESIS | |
Hb X and Hb A separate at alkaline pH; Hb X moves slower than Hb A on starch gel | |
CHROMATOGRAPHY | |
Hb X can be isolated on a DEAE-Sephadex column | |
STRUCTURE STUDIES | |
Tryptic digestion; separation of peptides by fingerprinting on paper and on a reversed phase HPLC column; amino acid analysis; sequencing | |
DNA ANALYSES | |
Mutation in the alpha2 gene: GAC->AAC at codon 64 (Ref. 6) | |
FUNCTIONAL STUDIES | |
Normal oxygen affinity | |
STABILITY | |
Normal | |
OCCURRENCE | |
Found in Filipino, Indian, and Rumanian families | |
OTHER INFORMATION | |
Quantity in heterozygotes 20-22%; found in combination with beta-thal, and with Hb D-Los Angeles | |
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REFERENCES |
1. | |
Blackwell, R.Q., Jim, R.T.S., Tan, T.G.H., Weng, M-I., Liu, C-S., and Wang, C-L.: Biochim. Biophys. Acta, 322:27, 1973. | |
2. | |
Bunn, H.F., Altman, A.J., Stangland, K., Firshein, S.I., Forget, B., Schmidt, G.J., and Jones, R.T.: Hemoglobin, 2:531, 1978. | |
3. | |
Baine, R.M., Wright, J.M., and Wilkinson, R.W.: Hemoglobin, 3:293, 1979. | |
4. | |
Harano, T., Harano, K., Ueda, S., Shibata, S., and Imai, K.: Hemoglobin, 5:591, 1981. | |
5. | |
Manca, L., Masala, B., Manca, M., Ferranti, P., and Pucci, P.: Hemoglobin, 18: 53, 1994. | |
6. | |
Landin, B. and Berg, A.: Hemoglobin, 18:71, 1994. | |