Hb Denmark Hill	alpha95(G2)Pro->Ala

CONTACT		alpha1beta2 contact
HEMATOLOGY		Normal in the heterozygote
ELECTROPHORESIS		Hb X moves slower than Hb A at alkaline pH; best separation with IEF
CHROMATOGRAPHY		Hb X was isolated by CM-Sephadex chromatography
STRUCTURE STUDIES		Tryptic digestion; separation of peptides by fingerprinting or reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation CCG->GCG; alpha2 or alpha1
FUNCTIONAL STUDIES		Increased oxygen affinity; decreased heme-heme interaction and Bohr effect
STABILITY		Not studied in detail
OCCURRENCE		Found in a West Indian female and a Spanish family
OTHER INFORMATION		Quantity in heterozygotes 15-19%

REFERENCES
1.		Wiltshire, B.G., Clark, K.G.A., Lorkin, P.A., and Lehmann, H.: Biochim. Biophys. Acta, 278:459, 1972.
2.		Vasseur, C., Francina, A., Wajcman, H., and Galacteros, F.: Hemoglobin, 15: 103, 1991.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.