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CONTACT | |
Heme contact | |
HEMATOLOGY | |
Normal in the heterozygote | |
ELECTROPHORESIS | |
Two bands, migrating slightly slower than Hb F and Hb A, were observed on IEF, but not by any other electrophoretic method | |
CHROMATOGRAPHY | |
No separation | |
STRUCTURE STUDIES | |
The alphaX, beta and alpha chains readily separate by reversed phase HPLC; tryptic digestion; separation of peptides by reversed phase HPLC; amino acid analysis; sequencing | |
DNA ANALYSES | |
A CTG->ATG mutation at codon 136 of the alpha2 gene (Ref. 3) | |
FUNCTIONAL STUDIES | |
Not reported | |
STABILITY | |
Normal | |
OCCURRENCE | |
Found in several Black families | |
OTHER INFORMATION | |
Observed in a patient with sickle cell anemia | |
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REFERENCES |
1. | |
Bowman, J.E., Bloom, R., Chen, S.S., Webber, B.B., Wilson, J.B., Kutlar, F., Kutlar, A., and Huisman, T.H.J.: Hemoglobin, 10:495, 1986. | |
2. | |
Gu, L-H., Wilson, J.B., Molchanova, T.P., McKie, K.M., McKie, V.C., and Huisman, T.H.J.: Hemoglobin, 17:295, 1993. | |
3. | |
Molchanova, T.P., Pobedimskaya, D.D., and Huisman, T.H.J.: Br. J. Haematol., 88:300, 1994. | |