Hb F-Calluna	Agamma12(A9)Thr->Arg

ELECTROPHORESIS		Hb FX moves between Hb S and Hb C at alkaline pH
IEF		Not reported
CHROMATOGRAPHY		Hb FX elutes between Hb A2 and Hb A on a DEAE-cellulose column
CHAIN SEPARATION		AgammaX = 10.7%; Ggamma = 71.5%; AgammaT = 17.8%
STRUCTURE STUDIES		Tryptic digestion of Hb FX; separation of peptides by reversed phase HPLC; amino acid analysis
DNA ANALYSES		Not reported; presumed mutation ACA->AGA at codon 12
NOTES		Found in a Caucasian baby; Hb FX 8.7% of total Hb or 10.8% of total Hb F; baby was also heterozygous for the AgammaT chain

REFERENCES
1.		Nakatsuji, T., Lam, H., and Huisman, T.H.J.: Hemoglobin, 7:563, 1983.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.