Hb Yusa	beta21(B3)Asp->Tyr

CONTACT		External
HEMATOLOGY		Normal in the heterozygote
ELECTROPHORESIS		Hb X and Hb A separate at alkaline pH; excellent separation by IEF
CHROMATOGRAPHY		Hb X and Hb A separate on an anion exchange column
STRUCTURE STUDIES		Tryptic digestion; fingerprinting; reversed phase HPLC: amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation GAT->TAT at codon 21
FUNCTION STUDIES		Normal oxygen affinity, Bohr, cooperativity, and 2,3-DPG effects
STABILITY		Normal
OCCURRENCE		Found in two Japanese families
OTHER INFORMATION		Quantity in heterozygotes ~41%

REFERENCES
1.		Harano, T., Harano, K., Ueda, S., Shibata, S., and Iuchi, I.: Hemoglobin, 5:121, 1981.
2.		Ohba, Y., Hattori, Y., Ami, M., Yagami, H., Miyaji, T., and Tani, Y.: Hemoglobin, 14:109, 1990.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.