Hb Minneapolis-Laos	beta118(GH1)Phe->Tyr

CONTACT		External
HEMATOLOGY		Normal in the heterozygote
ELECTROPHORESIS		IEF revealed an extra band with a mobility like Hb F
CHROMATOGRAPHY		Hb X and Hb A can be separated on a DEAE-cellulose or a DEAE-Sephadex column
STRUCTURE STUDIES		After aminoethylation, the betaX was digested with trypsin and the resulting fragments separated by reversed phase HPLC; amino acid analysis
DNA ANALYSES		Not reported; presumed mutation TTT->TAT at codon 118
FUNCTION STUDIES		Normal
STABILITY		Normal
OCCURRENCE		Found in a 27-year-old Laotian male and a 1-year-old Cambodian male and his mother
OTHER INFORMATION		Quantity in the heterozygote ~45%

REFERENCES
1.		Hedlund, B., Paine, S., Smith, C.M., II, Raines, J., Morrison, W.T., and Adams, J., III: Hemoglobin, 8:75, 1984.
2.		Kleman, K., Lubin, B., Kutlar, A., Wilson, J.B., Webber, B.B., and Huisman, T.H.J.: Hemoglobin, 11:401, 1987.

---

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.