Hb Prato	alpha31(B12)Arg->Ser

CONTACT		alpha1beta1 contact
HEMATOLOGY		Normal in the heterozygote
ELECTROPHORESIS		Hb X moves faster than Hb A at alkaline pH but does not separate at acidic pH
CHROMATOGRAPHY		Hb X separates from Hb A by DE-cellulose chromatography
STRUCTURE STUDIES		Tryptic digestion; separation of peptides by fingerprinting on paper or reversed phase HPLC; amino acid analysis
DNA ANALYSES		Not reported; presumed mutation AGG->AGC; alpha2 or alpha1
FUNCTIONAL STUDIES		Normal oxygen affinity, Bohr effect, and cooperativity
STABILITY		Mildly unstable
OCCURRENCE		Found in two Italian families
OTHER INFORMATION		Quantity in heterozygotes 20-30%

REFERENCES
1.		Marinucci, M., Mavilio, F., Massa, A., Gabbianelli, M., Fontanarosa, P.P., Camagna, A., Ignesti, C., and Tentori, L.: Biochim. Biophys. Acta, 578:534, 1979.
2.		De Marco, E.V., Crescibene, L., Pascua, A., Brancati, C., Bria, M., and Qualtieri, A.: Hemoglobin, 16:275, 1992.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.