Hb Poitiers	alpha45(CE3)His->Asp

CONTACT		External; heme contact
HEMATOLOGY		Normal in the heterozygote
ELECTROPHORESIS		Hb X separates from Hb A as a fast-moving band at alkaline pH; no separation at acidic pH; IEF is an excellent procedure to identify Hb X
CHROMATOGRAPHY		Hb X was isolated by DEAE-Sephadex chromatography
STRUCTURE STUDIES		Tryptic digestion of alphaA chain; separation of tryptic peptides by reversed phase HPLC; amino acid analysis; sequencing of the alphaT-6 peptide
DNA ANALYSES		Not reported; presumed mutation CAC->AAC; alpha2 or alpha1
FUNCTIONAL STUDIES		Increased oxygen affinity and autoxidation
STABILITY		Normal
OCCURRENCE		Found in three members of a French family
OTHER INFORMATION		Quantity in the heterozygote was ~27%

REFERENCES
1.		Bardakdjian, J., Kister, J., Wajcman, H., Boulard, P., Bohn, B., Blouquit, Y., and Galacteros, F.: Hemoglobin, 18:1, 1994.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.