Hb Mizushi	alpha75(EF4)Asp->Gly

CONTACT		External; near central cavity
HEMATOLOGY		Normal in the heterozygote
ELECTROPHORESIS		Hb X moves slower than Hb A at alkaline pH
CHROMATOGRAPHY		Not reported
STRUCTURE STUDIES		Tryptic digestion; separation of peptides by fingerprinting; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation GAC->GGC; alpha2 or alpha1
FUNCTIONAL STUDIES		Normal oxygen affinity, Bohr effect, and cooperativity
STABILITY		Normal
OCCURRENCE		Found in a Japanese family
OTHER INFORMATION		Quantity in heterozygotes ~14%

REFERENCES
1.		Iuchi, I., Shimasaki, S., Hidaka, K., Harano, T., Ueda, S., Shibata, S., Mizushima, J., and Kubo, N.: Hemoglobin, 4:209, 1980.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.