Hb Legnano	alpha141(HC3)Arg->Leu

CONTACT		C-terminus
HEMATOLOGY		Erythrocytosis in the heterozygote
ELECTROPHORESIS		Hb X moves ahead of Hb A on cellulose acetate at alkaline pH
CHROMATOGRAPHY		Not reported
STRUCTURE STUDIES		Tryptic digestion; separation of peptides by fingerprinting; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation CGT->CTT; alpha2 or alpha1
FUNCTIONAL STUDIES		Increased oxygen affinity; decreased cooperativity and Bohr effect
STABILITY		Slightly unstable
OCCURRENCE		Found in members of an Italian family
OTHER INFORMATION		Quantity in the heterozygote 32-35%

REFERENCES
1.		Mavilio, F., Marinucci, M., Tentori, L., Fontanarosa, P.P., Rossi, U., and Biagiotti, S.: Hemoglobin, 2:249, 1978.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.