Hb Kurosaki	alpha7(A5)Lys->Glu

CONTACT		External
HEMATOLOGY		Normal in the heterozygote
ELECTROPHORESIS		IEF demonstrated the presence of Hb X as a fast-moving band that focused anodally to Hb A (almost at the position of Hb H)
CHROMATOGRAPHY		Fast-eluting Hb by cation exchange HPLC; Hb X and Hb A were also separated on a DEAE-5PW HPLC column
STRUCTURE STUDIES		The alpha chain was isolated by CM-cellulose 8 M-urea-phosphate chromatography; tryptic peptides were separated by reversed phase HPLC; an alphaT-1,2 peptide was identified (alphaT-1 and alphaT-2 were absent) by amino acid analysis; sequence analysis
DNA ANALYSES		Not reported; presumed mutation AAG->GAG; alpha2 or alpha1
FUNCTIONAL STUDIES		Normal
STABILITY		Normal
OCCURRENCE		Found in a 70-year-old diabetic Japanese female
OTHER INFORMATION		Quantity in the heterozygote 17.7%

REFERENCES
1.		Harano, T., Harano, K., Imai, K., Murakami, T., and Matsubara, H.: Hemoglobin, 19:197, 1995.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.