Hb J-Rajappen	alpha90(FG2)Lys->Thr

CONTACT		External
HEMATOLOGY		Normal in the heterozygote
ELECTROPHORESIS		Hb X moves to the position of Hb J at alkaline pH
CHROMATOGRAPHY		Hb A and Hb X separate by cation and anion exchange HPLC
STRUCTURE STUDIES		Tryptic digestion; separation of peptides by fingerprinting; reversed phase HPLC; amino acid analysis; DNA sequencing
DNA ANALYSES		An AAG->ACG mutation at codon 90 in the alpha1-globin gene (Ref. 2)
FUNCTIONAL STUDIES		Not reported
STABILITY		Normal
OCCURRENCE		Found in members of Indian families
OTHER INFORMATION		Quantity in the heterozygote 19.5-20.5% (Ref. 2)

REFERENCES
1.		Hyde, R.D., Kinderlerer, J.L., Lehmann, H., and Hall, M.D.: Biochim. Biophys. Acta, 243: 515, 1971.
2.		Molchanova, T.P., Pobedimskaya, D.D., and Huisman, T.H.J.: Br. J. Haematol., 88:300, 1994.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.