Hb G-Waimanalo	alpha64(E13)Asp->Asn

ALSO KNOWN AS		Aida
CONTACT		External
HEMATOLOGY		Normal in the heterozygote
ELECTROPHORESIS		Hb X and Hb A separate at alkaline pH; Hb X moves slower than Hb A on starch gel
CHROMATOGRAPHY		Hb X can be isolated on a DEAE-Sephadex column
STRUCTURE STUDIES		Tryptic digestion; separation of peptides by fingerprinting on paper and on a reversed phase HPLC column; amino acid analysis; sequencing
DNA ANALYSES		Mutation in the alpha2 gene: GAC->AAC at codon 64 (Ref. 6)
FUNCTIONAL STUDIES		Normal oxygen affinity
STABILITY		Normal
OCCURRENCE		Found in Filipino, Indian, and Rumanian families
OTHER INFORMATION		Quantity in heterozygotes 20-22%; found in combination with beta-thal, and with Hb D-Los Angeles

REFERENCES
1.		Blackwell, R.Q., Jim, R.T.S., Tan, T.G.H., Weng, M-I., Liu, C-S., and Wang, C-L.: Biochim. Biophys. Acta, 322:27, 1973.
2.		Bunn, H.F., Altman, A.J., Stangland, K., Firshein, S.I., Forget, B., Schmidt, G.J., and Jones, R.T.: Hemoglobin, 2:531, 1978.
3.		Baine, R.M., Wright, J.M., and Wilkinson, R.W.: Hemoglobin, 3:293, 1979.
4.		Harano, T., Harano, K., Ueda, S., Shibata, S., and Imai, K.: Hemoglobin, 5:591, 1981.
5.		Manca, L., Masala, B., Manca, M., Ferranti, P., and Pucci, P.: Hemoglobin, 18: 53, 1994.
6.		Landin, B. and Berg, A.: Hemoglobin, 18:71, 1994.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.