Hb F-Hull | Agamma121(GH4)Glu->Lys |
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ELECTROPHORESIS | Hb FX moves slower than Hb C | ||
IEF | The same | ||
CHROMATOGRAPHY | Hb FX elutes first from a DEAE-cellulose column | ||
CHAIN SEPARATION | AgammaX = 11.5%; Ggamma = 72.5%; Agamma = 16% | ||
STRUCTURE STUDIES | Tryptic digestion of AE-gammaX chain; separation of peptides by fingerprinting; cation exchange HPLC; amino acid analysis | ||
DNA ANALYSES | A GAA->AAA mutation at codon 121 | ||
NOTES | Found in babies of English descent; quantity in heterozygotes 7-9% of total Hb F |
REFERENCES | |||
1. | Sacker, L.S., Beale, D., Black, A.J., Huntsman, R.G., Lehmann, H., and Lorkin, P.A.: Br. Med. J., 3:531, 1967. | ||
2. | Ahern, E.J., Ahern, V., Wiltshire, B.G., and Lehmann, H.: Biochim. Biophys. Acta, 303:242, 1973. | ||
3. | Nakatsuji, T., Burnley, M.S., and Huisman, T.H.J.: Blood, 66:803, 1985. |