Hb F-Malta-I	Ggamma117(G19)His->Arg

ELECTROPHORESIS		Hb FX moves between Hb S and Hb C at alkaline pH
IEF		About the same
CHROMATOGRAPHY		Hb FX can be separated from Hb A and Hb F by cation and anion exchange chromatography
CHAIN SEPARATION
STRUCTURE STUDIES		Tryptic digestion of AE-gammaX chain; separation of peptides by fingerprinting and reversed phase HPLC; amino acid analysis
DNA ANALYSES		A CAT->CGT mutation at codon 117 (Ref. 3)
NOTES		Occurs at reasonably high frequency in Maltese and some Italian newborn babies; its occurrence is linked to the beta chain variant Hb Valletta [beta87(F3)Thr->Pro] (Ref. 3)

REFERENCES
1.		Cauchi, M.N., Clegg, J.B., and Weatherall, D.J.: Nature, 223:311, 1969.
2.		Altay, G., Garver, F., Bannister, W.H., Grech, J.L., Felice, A.E., and Huisman, T.H.J.: Biochem. Genet., 15:915, 1977.
3.		Kutlar, F., Felice, A.E., Grech, J.L., Bannister, W.H., Kutlar, A., Wilson, J.B., Webber, B.B., Hu, H., and Huisman, T.H.J.: Hum. Genet., 86:591, 1991.

---

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.