Hb Olomouc	beta86(F2)Ala->Asp

CONTACT		External
HEMATOLOGY		Mild erythrocytosis in the heterozygote
ELECTROPHORESIS		Hb X and Hb A separate at alkaline pH; Hb X moves faster than Hb A; excellent separation by IEF; Hb X moves between Hb A and Hb F at acidic pH
CHROMATOGRAPHY		Hb X was isolated by DEAE-cellulose chromatography; Hb X elutes faster than Hb A on cation exchange HPLC columns
STRUCTURE STUDIES		Tryptic digestion of AE-betaX chain; separation of tryptic peptides by reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation GCC->GAC at codon 86
FUNCTION STUDIES		Slightly increased oxygen affinity
STABILITY		Stable
OCCURRENCE		Found in members of a Czechoslovakian family and a Japanese family
OTHER INFORMATION		Quantity in the heterozygote 46-47%

REFERENCES
1.		Indrak, K., Wiedermann, B.F., Batek, F., Wilson, J.B., Webber, B.B., Kutlar, A., and Huisman, T.H.J.: Hemoglobin, 11:151, 1987.
2.		Tagawa, Y., Fujinami, S., Kadota, Y., Nakagawa, T., Seki, T., Shiozaki, Y., Harano, T., Harano, K., and Ueda, S.: Hemoglobin, 16:73, 1992.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.