Hb Queens alpha34(B15)Leu->Arg
CONTACT alpha1beta1 contact
HEMATOLOGY Normal in the heterozygote
ELECTROPHORESIS Hb X separates from Hb A as a slowly-moving variant at alkaline pH; it also separates from Hb A at acidic pH; moves between Hb S and Hb F on cellulose acetate and starch gel; moves between Hb A and Hb S on agar gel
CHROMATOGRAPHY Hb X was isolated on a DEAE-cellulose column
STRUCTURE STUDIES Tryptic digestion; separation of peptides by fingerprinting or reversed phase HPLC; amino acid analysis
DNA ANALYSES Not reported; presumed mutation CTG->CGG; alpha2 or alpha1
FUNCTIONAL STUDIES Slightly increased oxygen affinity; normal Bohr effect and cooperativity
STABILITY Mildly unstable
OCCURRENCE Found in Korean, Chinese, Japanese, Vietnamese, and Thai families
OTHER INFORMATION Quantity in heterozygotes 10-16%
1. Tatsis, B.: Blood, 54:61a (Suppl. 1), 1979.
2. Zeng, Y-T., Huang, S-Z., Yen, Y-K., Li, W-C., Lam, H., Webber, B., Wilson, J.B., and Huisman, T.H.J.: Hemoglobin, 6:209, 1982.
3. Sugihara, J., Imamura, T., Yamada, H., Imoto, T., Matsuo, T., Sumida, I., and Yanase, T.: Biochim. Biophys. Acta, 701:45, 1982.
4. Yongsuwan, S., Svasti, J., and Fucharoen, S.: Hemoglobin, 11:567, 1987.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.