Hb Denmark Hill alpha95(G2)Pro->Ala
CONTACT alpha1beta2 contact
HEMATOLOGY Normal in the heterozygote
ELECTROPHORESIS Hb X moves slower than Hb A at alkaline pH; best separation with IEF
CHROMATOGRAPHY Hb X was isolated by CM-Sephadex chromatography
STRUCTURE STUDIES Tryptic digestion; separation of peptides by fingerprinting or reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES Not reported; presumed mutation CCG->GCG; alpha2 or alpha1
FUNCTIONAL STUDIES Increased oxygen affinity; decreased heme-heme interaction and Bohr effect
STABILITY Not studied in detail
OCCURRENCE Found in a West Indian female and a Spanish family
OTHER INFORMATION Quantity in heterozygotes 15-19%
1. Wiltshire, B.G., Clark, K.G.A., Lorkin, P.A., and Lehmann, H.: Biochim. Biophys. Acta, 278:459, 1972.
2. Vasseur, C., Francina, A., Wajcman, H., and Galacteros, F.: Hemoglobin, 15: 103, 1991.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.