Hb F-Beech Island	Agamma53(D4)Ala->Asp

ELECTROPHORESIS		Hb FX moves slightly faster than Hb A at alkaline pH
IEF		Not reported
CHROMATOGRAPHY		Hb FX elutes together with minor Hb F1 in DEAE-cellulose chromatography
CHAIN SEPARATION		AgammaX = 12%; Ggamma = 74.1%; Agamma = 13.9%
STRUCTURE STUDIES		Tryptic digestion of gammaX chain; separation of peptides by reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation GCC->GAC at codon 53
NOTES		Found in a few Caucasian and Black babies in the SE-USA; quantity of Hb FX estimated at 15-16%

REFERENCES
1.		Chen, S.S., Wilson, J.B., Webber, B.B., and Huisman, T.H.J.: Hemoglobin, 9: 525, 1985.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.