| 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | |
| 0 | Leu->Ile Gln->Lys | Lys->Glu Ile->Asn Gln->Glu Asn->Asp | Lys->Met | Pro->Thr | Gln->His | |||||
| 10 | Ser->Pro | Thr->Ile | Thr->Asn | Gly->Ala Val->Leu Val->Ile Asp->Glu Ser->Thr Asn->Lys | Leu->Gln Ile->Lys | Val->Asp Ala->Ser Phe->Tyr Pro->Leu Ser->Cys | Ile->Met Leu->Met | His->Arg | ||
| 20 | Asp->His | Asn->His | Leu->His | Met->Arg | His->Tyr Ala->Pro Ser->Leu Ser->Ile | Ser->Asn Thr->Lys | Lys->Arg Gln->Arg Ala->Val | |||
| 30 | Val->Glu Gly->Ser Ala->Thr Arg->Trp Thr->Met | Pro->Gln | Val->Met | Leu->Phe Ile->Phe | ||||||
| 40 | Pro->His | Gly->Val | Leu->Arg Ile->Arg | Ala->Asp Cys->Phe | Gly->Cys | Val->Phe Asp->Tyr | Asn->Tyr | |||
| 50 | Cys->Arg | Thr->Arg | Gly->Asp Ala->Glu | Pro->Arg | ||||||
| 60 | Cys->Tyr Ser->Phe | Ser->Arg | ||||||||
| 70 | Gly->Glu | Leu->Trp | Ser->Tyr | |||||||
| 80 | Cys->Trp | |||||||||
| 90 | Gly->Arg Ser->Trp | |||||||||
| 120 | Gly->Trp |
The mass of normal alpha chain is 15126.3 Da and that of the normal beta chain 15867.5, the changes induced by single point mutations are given above (only those allowed by the genetic code are given). As an example the change from Asp to His will increase the normal mass by 22 units and the reverse change will decrease the mass by the same amount.